Estimating groundwater recharge is one of the important challenges in groundwater resource management. Various techniques have been developed in recent years to estimate aquifer recharge. The water level fluctuation (WTF) method is one of the simplest and most practical of these techniques. In this study, the recharge rate of the Jiroft Plain aquifer was estimated by examining groundwater level fluctuations over a 24-year period. The results of this study showed that the minimum, average, and maximum annual aquifer recharge during this period were 0.97, 51.18, and 186.58 mm, respectively. The lowest recharge rate occurred in the southern part of the aquifer, while the highest recharge rate occurred in its northeastern margin. Factors such as surface geomorphological features, soil texture, and characteristics of the unsaturated zone have influenced the spatial distribution of aquifer recharge. Hydrological drought has a significant impact on aquifer recharge. Additionally, increased aquifer pumping due to the expansion of agricultural fields has also contributed to an increase in aquifer recharge. However, this increased recharge has not compensated for the water table drawdown resulting from aquifer pumping, and the water level continues to decline.