Wastewater from various industries is one of the main sources of environmental pollution, which has detrimental effects on the environment and the lives of living organisms. In recent years, adsorption methods using adsorbents to remove contaminants from water resources have been abundant. Solfounate and Pentaaza Tetra Ethylene supported Polyacrylamide (PAM-SO3-N5) as a novel adsorbent can be effective for removal of chemical pollutants of the aquatic solution. The purpose of this research is removal of Kalmagate using PAM-SO3-N5 as an adsorbent from polluted water in vitro. The effects of variables such as pH, contact time, initial concentration and adsorbent amount were observed to reach best adsorption conditions. Isotherms of Langmuir, Freundlich and Temkin have been fitted with the data of experiment. In addition, kinetics of pseudo- first order, pseudo- second order, intra-particle diffusion and Elovich were also fitted with the experiment data. Also, the results indicated that the best conditions for removal of Kalmagate dye were: pH= 2, removing time= 60 minutes, adsorbent dosage= 0.014 g and initial concentration of dye= 360 mg L-1. For PAM-SO3-N5, Freundlich isotherms showed a good agreement with the experimental data. Using this model to maximize absorption capacity of 1732.5 (mg g-1) for PAM-SO3-N5. Absorption rates showed a quick responses which was less than one hours. Based on these results, the adsorption kinetics of pseudo- second- order was more consistent with the experimental data (R2=0.97). The results show that PAM-SO3-N5 absorbent is effective in removing Kalmagate contaminants from the aqueous solutions due to its high