ارزیابی روش کاربرد EDTA در کاهش ریسک آبشویی سرب به آب‌های زیرزمینی با استفاده از Hordeum bulbosum L.

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، دانشکده آب و خاک، گروه مرتع و آبخیزداری، دانشگاه زابل

2 بیابان‌زدایی، دانشکده آب و خاک، گروه مرتع و آبخیزداری، دانشگاه زابل

چکیده

مطالعه حاضر به­منظور افزایش کارایی گیاه­استخراجی گونه Hordeum bulbosum L.)جو پیاردار) در خاک­های (بافت خاک لومی رسی) آلوده به سرب بااستفاده از اتیلن دی امین تترا استیک اسید (EDTA) (غلظت 5/1، 5، 10 میلی­مول در کیلوگرم)، تعیین بهترین زمان تیمار و شیوه کاربرد EDTA جهت کاهش خطر آبشویی سرب به آب­های زیرزمینی انجام شد. از خاک­های غیرآلوده بدون EDTA و آلوده بدون EDTA به­عنوان شاهد استفاده گردید. نتایج به­دست آمده نشان داد که در تیمار 5EDTA حداکثر (70/890 میلی­گرم در کیلوگرم در اندام­های زیرزمینی و 65/720 میلی­گرم در کیلوگرم در اندام­های هوایی) میزان برداشت سرب صورت گرفت؛ بنابراین در مرحله دوم تحقیق تیمار 5 میلی­مول در کیلوگرم جهت ارزیابی بهترین زمان برداشت گیاه در دوره­های زمانی 20 روز (رشد رویشی)، 80 روز (مرحله گلدهی) 140 روز (مرحله بذردهی) استفاده شد. نتایج نشان داد که با گذشت زمان، غلظت سرب در بافت­های گیاهی افزایش داشت، اما بین مرحله گلدهی و بذردهی تفاوت معنی­دار وجود نداشت (p‹0/05) و بهترین زمان برداشت گیاه، به­منظور دستیابی به حداکثر برداشت سرب از خاک دوره گلدهی است. در مرحله سوم جهت کاهش خطر آبشویی سرب، غلظت 5 میلی­مول در کیلوگرم در سه روش یک­بار، دوبار متوالی و سه­بار متوالی به خاک اضافه شد. نتایج نشان داد که تحت کاربرد EDTA به­صورت یک­بار، میزان سرب خاک به حداقل رسید. داده­ها نشان داد که حداکثر میزان سرب در اندام­های گیاهی در کاربرد روش یک­بار به­دست آمد، درحالی­که غلظت فلز در اندام­های گیاهی بین روش دوبار و سه­بار متوالی تفاوت قابل ملاحظه­ای وجود نداشت. به­طورکلی، بهینه گیاه­استخراجی گونه H. bulbosum و کاهش خطر آبشویی سرب در کاربرد غلظت 5 میلی­مول در کیلوگرم EDTA با روش یک­باراضافه کردن و برداشت گیاه 80 روز بعد از کاشت به­دست آمد.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of EDTA application method to reduce Pb leaching risk into ground waters using Hordeum bulbosum L.

نویسندگان [English]

  • Mahdiyeh Ebrahimi 1
  • Nasrollah Aslinezhad 2
1 Assistant Professor, University of Zabol
2 M. Sc Student of Combating Desertification, University of
چکیده [English]

This study was conducted to increase phytoextraction efficiency of Hordeum bulbosum L. in lead contaminate soil (Loamy Clay) in the EDTA-assisted, Ethylen ediamine tetra acetic acid (1.5, 5, 10 mmol kg−1), assessing optimum of treatment time and method of EDTA application to reduce Pb leaching risk into ground waters. Uncontaminated soil without EDTA (C) and contaminated soil without EDTA (W) used as the controls. The results revealed that the greatest Pb uptake (890.70 mgKg-1 in underground organs and 720.65 mgkg-1 in above ground organs) was observed in 5EDTA treatment. Therefore, 5mmolkg-1 was used in second step for assessing harvest time for 20(Vegetative stage), 80 (Flowering stage) and 140(Seed production) days. Results showed that the concentration of Pb in plant tissues was increased with passage of time, but between flowering stage and seed production there was no significant difference (p‹0/05) and the best harvest time in order to achieve maximum removal of the metal was flowering stage. In third step to reduce leaching risk of Pb-EDTA, 5 mmolkg-1 EDTA in three ways of single, double (two successive) and triple (three successive) were added to the soil. The results showed that under single dosage application, Pb content in the soil reached at its minimum concentration. The data indicated that the maximum Pb concentration in the plant organs was calculated at the single dosage while metal concentration in the plant organs did not vary considerably when double and triple dosage were added. Overall, optimum phytoextraction of H. bulbosum and Pb leaching risk reduction was observed when 5 mmol kg−1 EDTA was added in single dosage, 80 days after the plant cultivation

کلیدواژه‌ها [English]

  • heavy metals
  • EDTA
  • Phytoextraction
  • Hordeum bulbosum L

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